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| ELISA kit for measuring Aspergillus versicolor antigen. | ||||
| Sensitivity: | 2U/ml | |||
| Limit of Detection: | 16U/ml | |||
| Assay Performance: | Inquire | |||
| Contents: | Monoclonal antibody 5F5, Aspergillus versicolor Standard, Biotinylated antibody 9A5; sufficient reagents for 10 microtiter plates. | |||
| Storage: | Store at 4°C. | |||
| Not Provided: | 50mM carbonate/bicarbonate buffer, pH 9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine serum albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2 | |||
| Notes: | ||||
| Product Resources: | EL-AVX Certificate of Analysis | |||
| For research and commercial use in vitro: not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 5F5 (clone 5F5 H2 H8) | |||
| Vial: | Red Cap; 100μl | |||
| Immunogen: | Aspergillus versicolor extract | |||
| Isotype: | Mouse IgG2A | |||
| Specificity: | Binds to a species specific epitope of Aspergillus versicolor antigen. | |||
| Purification: | Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Concentration: | 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG(1.42=1mg/ml), 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 9A5 (clone 9A5 B9 C10) | |||
| Vial: | Brown Cap; 100μl | |||
| Immunogen: | Aspergillus versicolor extract | |||
| Isotype: | Mouse IgG2A | |||
| Specificity: | Binds to a species specific epitope of Aspergillus versicolor antigen. | |||
| Purification: | Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Antigen Standard: | Aspergillus versicolor | |||
| Vial: | White Cap; 400μl | |||
| Composition: | Aspergillus versicolor source material extract prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4. | |||
| Concentration: | 10,000U/ml AveX | |||
| Calibration: | The concentration of the extract was measured using a total protein assay. The extract is calibrated in arbitrary units where 1 unit of AveX equals approximately 1 nanogram of total protein. | |||